crc cell lines colo201  (Fukui Bank Ltd)

Journal: Cancer Cell International

Article Title: Enhanced anti-tumor effects by combination of tucatinib and radiation in HER2-overexpressing human cancer cell lines

doi: 10.1186/s12935-024-03458-3

Figure Lengend Snippet: Metabolic activity, proliferation, apoptosis and necrosis of CRC and NSCLC cell lines. CRC cell lines (LS411N, DLD-1, COLO201) and NSCLC cell lines (DV90, NCI-H1781, A549) 72 h after treatment with TUC and/or single-dose IR. Data are presented as mean ± SEM of one experiment per cell line ( n = 3). Significant differences relative to the corresponding 0 nM TUC group are indicated by asterisks between groups marked with a hash symbol. ( A ) Metabolic activity, measured by WST1 assay, and ( B ) proliferation, measured by BrdU incorporation assay, are presented relative to untreated non-irradiated control samples (= 1). Experiments were performed in duplicate (A, NCI-H1781) or triplicate (all others). ( C ) Apoptotic (plain) and necrotic (hatched) fractions, measured by Annexin V/PI assay, are shown together with one representative dot plot per entity. Asterisks and hash symbols indicate significant differences in apoptotic fractions

Article Snippet: CRC cell lines COLO201 and DLD1 were also provided by PD Dr. C. Stäubert, while LS411N was purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Activity Assay, BrdU Incorporation Assay, Irradiation, Control

Journal: Cancer Cell International

Article Title: Enhanced anti-tumor effects by combination of tucatinib and radiation in HER2-overexpressing human cancer cell lines

doi: 10.1186/s12935-024-03458-3

Figure Lengend Snippet: ( A ) Metabolic activity of BC, CRC and NSCLC cell lines with different PI3K and HER2 statuses after combined treatment with ALP/TUC. Metabolic activity, measured by WST1 assay, of CRC cell lines DLD1 and COLO201, NSCLC cell line NCI-H2030, and BC cell lines HCC1954 and MCF7 72 h after treatment with ALP and/or TUC. Relative values (untreated control = 1) of a single experiment performed in triplicate are presented as mean ± SEM. Cell lines are marked by shading and color as either HER2-overexpressing (hatched) or HER2-negative (plain) as well as PI3KCA-mutated (red coloring) or PI3KCA-wild type (yellow coloring). (B) , ( C ), ( D ) Verification of HER2 expression by Immmunofluorescence in cells used for the TUC/ALP combinatorial treatments. ( B ) Difference in mean fluorescence intensities of HER2 and IgG negative control are presented in five cell lines. Data from two independent experiments are presented as mean ± SEM. ( C ) Representative histogram overlays of HER2 staining versus IgG negative control for each cell line. ( D ) Representative photographs of HER2-(Alexa488, green) and nuclear (DAPI, blue) stained floating cells prepared for flow cytometry analysis (scale bar 50 μm)

Article Snippet: CRC cell lines COLO201 and DLD1 were also provided by PD Dr. C. Stäubert, while LS411N was purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Activity Assay, Control, Expressing, Fluorescence, Negative Control, Staining, Flow Cytometry

Journal: Cancer Cell International

Article Title: Enhanced anti-tumor effects by combination of tucatinib and radiation in HER2-overexpressing human cancer cell lines

doi: 10.1186/s12935-024-03458-3

Figure Lengend Snippet: PIK3CA and HER2 status in five cancer cell lines

Article Snippet: CRC cell lines COLO201 and DLD1 were also provided by PD Dr. C. Stäubert, while LS411N was purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Over Expression

Journal: Translational and Regulatory Sciences

Article Title: Suppression of tensin 2 promotes intestinal tumorigenesis by liberating integrin-linked kinase-induced nuclear translocation of β-catenin

doi: 10.33611/trs.2020-009

Figure Lengend Snippet: Fig. 1. Tns2 suppresses the proliferation and migration of colorectal cancer cell lines. (A) RT-qPCR analysis showed that TNS2 mRNA expression level was lower in HCT116 cells than in Colo201 and SW620 cells. In Colo201 and SW620 cells, siRNA treatment sup- pressed TNS2 mRNA expression. Relative fold changes in TNS2 expression were calculated using the comparative 2−ΔΔCT method and normalized to TNS2 level from HCT116 cells. (B) RT-PCR analysis showed the stable expression of murine Tns2 in HCT116 cells. Minus or plus indicates the absence or presence of pCAG-Tns2, respectively. (C–E) Proliferation of colorectal cancer cell lines in the presence or absence of TNS2 gene. TNS2 KD significantly increased the proliferation of Colo201 (C) and SW620 cells (D) (n=3). Tns2 overexpression induced a significant decrease in HCT116 cell proliferation (E) (n=4). (F–H) Migration of colorectal cancer cell lines in the presence or absence of TNS2 gene. TNS2 KD significantly increased the migration of Colo201 (F) and SW620 (G) cells (n=4). Tns2 overexpression induced a significant decrease in HCT116 cell migration (H) (n=4). Values are means ± SD. *P<0.05, ** P<0.01, ***P<0.001.

Article Snippet: Human CRC cell lines Colo201, SW620, and HCT116 were purchased from the American Type Culture Collection (Manassas, VA, USA) and cultured in Roswell Park Memorial Institute (RPMI)-1640 medium (Nacalai Tesque, Kyoto, Japan), Leibovitz’s L-15 medium (Wako, Osaka Japan), and Dulbecco’s modified Eagle’s medium (DMEM; Nacalai Tesque) supplemented with 10% fetal bovine serum (HyClone, Logan, UT, USA), 100 units/ml of penicillin, and 100 μg/ml of streptomycin, respectively.

Techniques: Migration, Quantitative RT-PCR, Expressing, Reverse Transcription Polymerase Chain Reaction, Over Expression

Journal: Translational and Regulatory Sciences

Article Title: Suppression of tensin 2 promotes intestinal tumorigenesis by liberating integrin-linked kinase-induced nuclear translocation of β-catenin

doi: 10.33611/trs.2020-009

Figure Lengend Snippet: Fig. 2. Tns2 suppresses the Wnt/β-catenin activity and nuclear translocation of β-catenin. (A)TNS2 KD increases Wnt/β-catenin activity in Colo201 cells, as detected by the TOPFlash luciferase assay (n=6). (B) Tns2 overexpression decreases Wnt/β-catenin activity in HCT116 cells, as detected by the TOPFlash luciferase assay (n=6). (C) β-catenin level was similar between the whole cell lysates of HCT116-Tns2 cells and control cells. (D) Tns2 overexpression decreased the nuclear translocation of β-catenin in HCT116 cells, as detected by immunofluorescence (Control: n=221, Tns2: n=117). White arrows indicate the position of the nucleus. Values are means ± SD. *P<0.05, **P<0.01.

Article Snippet: Human CRC cell lines Colo201, SW620, and HCT116 were purchased from the American Type Culture Collection (Manassas, VA, USA) and cultured in Roswell Park Memorial Institute (RPMI)-1640 medium (Nacalai Tesque, Kyoto, Japan), Leibovitz’s L-15 medium (Wako, Osaka Japan), and Dulbecco’s modified Eagle’s medium (DMEM; Nacalai Tesque) supplemented with 10% fetal bovine serum (HyClone, Logan, UT, USA), 100 units/ml of penicillin, and 100 μg/ml of streptomycin, respectively.

Techniques: Activity Assay, Translocation Assay, Luciferase, Over Expression, Control, Immunofluorescence